Bio-One

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Business Services
Commercial Cleaning

Bio-One

Business Services
Commercial Cleaning

Bio-One Franchise FDD, Costs & Fees (2025)

Bio-One provides compassionate biohazard cleaning services, helping communities handle sensitive and hazardous situations with professionalism and care.

Bio-One Inc. is a leading franchise specializing in biohazard and decontamination cleanup services. Founded in 2006 and headquartered in Greenwood Village, Colorado, Bio-One began franchising in 2010. 

The company focuses on providing essential services such as crime scene cleanup, hoarding remediation, and biohazard decontamination, addressing some of the most challenging and sensitive situations with discretion, care, and compassion. 

Bio-One differentiates itself by not only being the first franchise in this niche but also by maintaining a steadfast commitment to its mission of "Help First, Business Second."

Initial investment

Here's what you would need to invest if you were to start this franchise. These costs are provided by the franchisor in the Franchise Disclosure Document.

Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795
Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795

Franchise Disclosure Document

KEY FRANCHISE STATS

All you need to know about this franchise

Started franchising
2011
Franchisees

?

130
+
8%
8%
Franchise fee

?

$60,000
Investment

?

$116,000 - $170,000
Revenue (AUV)

?

Undisclosed
Upgrade

$423,000

+
n.a.
n.a.
Operating Profit

?

Upgrade

n.a.

Home based

?

Yes
Upgrade
Yes
Passive investment

?

No
Upgrade
No
FDD
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Competitors

Below are some of

Bio-One

key competitors in the

Commercial Cleaning

sector.

Franchise
Franchisees
Initial fee
Total investment
Revenue
Upgrade
Gross Profit
Upgrade
EBITDA
Upgrade

130

New
+
8%
8%

$60,000

$116,000

$170,000

n.a.

$423,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

20

New
+
11%
11%

$29,750

$44,000

$61,000

n.a.

$306,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

330

New
+
-3%
-3%

$27,000 - $40,700

$85,000

$114,000

n.a.

$753,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

1060

New
+
31%
31%

$33,000

$40,000

$53,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

2

New
+
-71%
-71%

$10,000 - $38,000

$11,000

$223,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

44

New
+
n.a.
n.a.

$28,500 - $62,000

$58,000

$939,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

45

New
+
2%
2%

$75,000

$110,000

$355,000

n.a.

$3,206,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

103

New
+
-9%
-9%

$40,000

$126,000

$300,000

n.a.

$387,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

448

New
+
-7%
-7%

$14,900

$140,000

$197,000

n.a.

$759,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

70

New
+
-26%
-26%

$20,000

$86,000

$129,000

n.a.

$103,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

419

New
+
1%
1%

$36,000

$56,000

$110,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

5588

New
+
-6%
-6%

$40,320

$18,000

$64,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

1099

New
+
-24%
-24%

$36,000

$101,000

$265,000

n.a.

$218,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

45

New
+
7%
7%

$98,000

$219,000

$339,000

n.a.

$2,485,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

615

New
+
-15%
-15%

$2,500 - $72,000

$4,000

$134,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

58

New
+
-30%
-30%

$45,000

$129,000

$151,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

131

New
+
2%
2%

$59,900

$169,000

$244,000

n.a.

$546,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

29

New
+
190%
190%

$59,500

$95,000

$141,000

n.a.

$889,000

$xxx,xxx

47.0%

xx%

n.a.

n.a.

xx%

n.a.

238

New
+
-3%
-3%

$45,000

$106,000

$131,000

n.a.

$375,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

1517

New
+
4%
4%

$0.5 per household

$82,000

$160,000

n.a.

$83,000

$xxx,xxx

34.7%

xx%

n.a.

14.2%

xx%

n.a.

210

New
+
-2%
-2%

$100,000

$158,000

$522,000

n.a.

$1,196,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

59

New
+
13%
13%

$49,000

$199,000

$253,000

n.a.

$240,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

24

New
+
-11%
-11%

$30,000

$52,000

$83,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

2202

New
+
12%
12%

$90,000

$241,000

$302,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

48

New
+
7%
7%

$65,000

$108,000

$149,000

n.a.

$432,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

94

New
+
11%
11%

$50,000

$96,000

$220,000

n.a.

$1,021,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

56

New
+
2%
2%

$10,000 to $75,000

$148,000

$462,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

69

New
+
3%
3%

$25,000

$43,000

$59,000

n.a.

$265,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

269

New
+
2%
2%

$59,900

$107,000

$174,000

n.a.

$560,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

221

New
+
6%
6%

$20,000

$82,000

$173,000

n.a.

$1,405,000

$xxx,xxx

38.0%

xx%

n.a.

n.a.

xx%

n.a.

24

New
+
0%
0%

$74,500

$165,000

$195,000

n.a.

$504,000

$xxx,xxx

94.6%

xx%

n.a.

n.a.

xx%

n.a.

41

New
+
78%
78%

$40,000

$86,000

$263,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

117

New
+
29%
29%

$19,950

$93,000

$150,000

n.a.

$567,000

$xxx,xxx

53.8%

xx%

n.a.

n.a.

xx%

n.a.

117

New
+
2%
2%

$8,000

$11,000

$78,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

74

New
+
-15%
-15%

$6,999

$36,000

$107,000

n.a.

$399,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

20

New
+
100%
100%

$38,000

$127,000

$223,000

n.a.

$149,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

148

New
+
2%
2%

$45,000

$80,000

$127,000

n.a.

$405,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

158

New
+
-19%
-19%

$3,000

$16,000

$418,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

0

New
+
n.a.
n.a.

$50,000

$153,000

$176,000

n.a.

$0

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

933

New
+
-6%
-6%

$55,000

$127,000

$166,000

n.a.

$788,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

5

New
+
n.a.
n.a.

$49,000

$198,000

$264,000

n.a.

$69,000

$xxx,xxx

64.4%

xx%

n.a.

12.9%

xx%

n.a.

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Number of units

Training 

Bio-One Franchise offers comprehensive training and support to ensure franchisees are well-prepared to operate successfully in the biohazard and decontamination cleanup industry. The training program is designed to equip franchisees with the necessary skills, knowledge, and resources to handle the sensitive and demanding nature of the work.

Initial Training

Bio-One provides an intensive initial training program at their corporate headquarters. This program typically lasts several days and covers a broad range of topics essential for running a Bio-One franchise. The initial training includes:

  1. Operational Procedures: Franchisees learn the standard operating procedures for biohazard cleanup, crime scene cleaning, hoarding remediation, and other decontamination services. This ensures that they can perform these tasks efficiently and safely.
  2. Safety Protocols: Given the nature of the work, safety is paramount. Bio-One trains franchisees on all necessary safety protocols, including the use of personal protective equipment (PPE), proper handling and disposal of hazardous materials, and adherence to OSHA guidelines.
  3. Customer Service: Handling emotionally sensitive situations is a core part of Bio-One’s services. The training includes strategies for interacting with clients compassionately and professionally during their most difficult times.
  4. Business Management: Franchisees are instructed on the business aspects of running a Bio-One franchise, including financial management, marketing strategies, and operational logistics. This helps ensure that franchisees are not only proficient in the cleanup services but also in managing their business effectively.

Territory Protection

Bio-One offers territory protection to its franchisees, ensuring that each franchisee has a defined and exclusive geographic area to operate within. This territorial exclusivity allows franchisees to develop their business without the concern of direct competition from other Bio-One franchisees in their immediate market. 

The protected territory is designed to provide enough potential clientele to support a thriving business, taking into account the population density and demand for biohazard cleanup services.

By offering this level of territorial protection, Bio-One helps franchisees establish a strong market presence and build lasting relationships with clients and local authorities. 

Competitors

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Franchisees

?

74
+
-15%
-15%
Investment

?

$36,000 - $107,000
Business Services
Commercial Cleaning

Renue

Renue is a commercial cleaning and sanitization franchise specializing in deep cleaning and surface restoration for hotels, healthcare facilities, and offices.

Franchisees

?

24
+
0%
0%
Investment

?

$165,000 - $195,000
Business Services
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Buildingstars

Buildingstars provides commercial cleaning and janitorial services, focusing on quality and customer satisfaction to maintain clean and professional workspaces.

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+
31%
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Investment

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$40,000 - $53,000
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+
n.a.
n.a.
Investment

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ecomaids

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58
+
-30%
-30%
Investment

?

$129,000 - $151,000
Business Services
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20
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11%
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Investment

?

$44,000 - $61,000
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