Create a free account to access this table
Sign up for free
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
2,500+ franchises
Access our entire database: 2,500+ US franchises, updated every day
Essential financial data
Royalty fees, franchise fees, investment costs and more
View 1 FDD
Our free plan allows you to access 1 FDD. Upgrade to Pro or purchase the FDD to download

Bio-One Franchise FDD, Costs & Fees (2025)

Bio-One provides compassionate biohazard cleaning services, helping communities handle sensitive and hazardous situations with professionalism and care.

Bio-One Inc. is a leading franchise specializing in biohazard and decontamination cleanup services. Founded in 2006 and headquartered in Greenwood Village, Colorado, Bio-One began franchising in 2010. 

The company focuses on providing essential services such as crime scene cleanup, hoarding remediation, and biohazard decontamination, addressing some of the most challenging and sensitive situations with discretion, care, and compassion. 

Bio-One differentiates itself by not only being the first franchise in this niche but also by maintaining a steadfast commitment to its mission of "Help First, Business Second."

Initial investment

Here's what you would need to invest if you were to start this franchise. These costs are provided by the franchisor in the Franchise Disclosure Document.

Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795
Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795

Bio-One

2024

Franchise Disclosure Document

Number of units

Training 

Bio-One Franchise offers comprehensive training and support to ensure franchisees are well-prepared to operate successfully in the biohazard and decontamination cleanup industry. The training program is designed to equip franchisees with the necessary skills, knowledge, and resources to handle the sensitive and demanding nature of the work.

Initial Training

Bio-One provides an intensive initial training program at their corporate headquarters. This program typically lasts several days and covers a broad range of topics essential for running a Bio-One franchise. The initial training includes:

  1. Operational Procedures: Franchisees learn the standard operating procedures for biohazard cleanup, crime scene cleaning, hoarding remediation, and other decontamination services. This ensures that they can perform these tasks efficiently and safely.
  2. Safety Protocols: Given the nature of the work, safety is paramount. Bio-One trains franchisees on all necessary safety protocols, including the use of personal protective equipment (PPE), proper handling and disposal of hazardous materials, and adherence to OSHA guidelines.
  3. Customer Service: Handling emotionally sensitive situations is a core part of Bio-One’s services. The training includes strategies for interacting with clients compassionately and professionally during their most difficult times.
  4. Business Management: Franchisees are instructed on the business aspects of running a Bio-One franchise, including financial management, marketing strategies, and operational logistics. This helps ensure that franchisees are not only proficient in the cleanup services but also in managing their business effectively.

Territory Protection

Bio-One offers territory protection to its franchisees, ensuring that each franchisee has a defined and exclusive geographic area to operate within. This territorial exclusivity allows franchisees to develop their business without the concern of direct competition from other Bio-One franchisees in their immediate market. 

The protected territory is designed to provide enough potential clientele to support a thriving business, taking into account the population density and demand for biohazard cleanup services.

By offering this level of territorial protection, Bio-One helps franchisees establish a strong market presence and build lasting relationships with clients and local authorities. 

Competitors

Below are some of

Bio-One

key competitors in the

Commercial Cleaning

sector.

Franchise
Franchisees
Initial fee
Total investment
Revenue
Sign up
Gross Profit
Sign up
EBITDA
Sign up

130

New
+
8%
8%

$60,000

$116,000

$170,000

n.a.

$423,000

$xxx,xxx

n.a.

xx%

n.a.

n.a.

xx%

n.a.

Competitors

Cleanest Restaurant Group

Cleanest Restaurant Group is a restaurant cleaning service franchise offering specialized front- and back-of-house deep cleaning, serving full-service and quick-service restaurants, and known for niche expertise, proprietary cleaning solutions, low-competition territories, and recurring nighttime service contracts.

Franchisees

?

10
+
900%
900%
Investment

?

$103,000 - $144,000

Cleannet

CleanNet is a commercial cleaning franchise offering janitorial and facility-maintenance services to offices and industrial properties, serving business clients and operators, and known for proprietary systems, national account support, and scalable operations.

Franchisees

?

182
+
6%
6%
Investment

?

$20,000 - $85,000

You've Got Maids

You've Got Maids is a residential cleaning franchise providing professional maid services with a focus on eco-friendly practices and exceptional customer service.

Franchisees

?

74
+
-15%
-15%
Investment

?

$36,000 - $107,000

Renue

Renue is a commercial cleaning and sanitization franchise specializing in deep cleaning and surface restoration for hotels, healthcare facilities, and offices.

Franchisees

?

24
+
0%
0%
Investment

?

$165,000 - $195,000

Buildingstars

Buildingstars provides commercial cleaning and janitorial services, focusing on quality and customer satisfaction to maintain clean and professional workspaces.

Franchisees

?

1060
+
31%
31%
Investment

?

$40,000 - $53,000

Sparkle Squad

Sparkle Squad provides residential and commercial window cleaning services. Their eco-friendly solutions and professional techniques leave windows spotless and sparkling.

Franchisees

?

0
+
n.a.
n.a.
Investment

?

$153,000 - $176,000

Our latest newsletters

Franchising 101

Is This Franchise the Right Fit for You?

December 15, 2025
Due Diligence & Decision-Making

Choosing a franchise goes beyond reviewing fees and financials — it requires an honest assessment of your goals, skills, and risk tolerance. This article outlines the key questions prospective owners should ask before committing. It helps clarify whether the opportunity aligns with your lifestyle, long-term plans, and operational preferences.

Franchisee Validation: Interviews, Reference Checking, Site Visits

December 15, 2025
Due Diligence & Decision-Making

Speaking with current and former franchisees is one of the most reliable ways to understand how a franchise really operates. This guide explains how to conduct validation calls, what questions to ask, and how to identify patterns in feedback. It provides a practical framework for uncovering real performance expectations and support quality.

How to Evaluate a Franchise Opportunity (Industry, Brand Strength, Market Fit)

December 15, 2025
Due Diligence & Decision-Making

Not all franchise opportunities offer the same potential. This article breaks down how to evaluate a brand’s industry, financial health, support systems, and competitive positioning. It helps prospective buyers compare options and identify franchises with strong long-term viability.

Building a Franchise Business Plan & Pro Forma Financials

December 15, 2025
Costs, Fees and Financing

A clear, well-structured business plan is essential for securing financing and preparing for the realities of franchise ownership. This article explains how to build a plan that covers market analysis, operations, staffing, and local marketing. It also outlines how to create realistic pro forma financials, including revenue projections, startup costs, and break-even analysis. Readers gain a practical framework they can use when applying for loans or planning their first years of operations.