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Bio-One Franchise FDD, Costs & Fees (2026)

Bio-One provides compassionate biohazard cleaning services, helping communities handle sensitive and hazardous situations with professionalism and care.

KEY FRANCHISE STATS

Franchisees

?

130
+
8%
8%
Franchise fee

?

$60,000
Investment

?

$116,000 - $170,000
Revenue (AUV)

?

Undisclosed

$423,000

+
n.a.
n.a.
Upgrade

Bio-One Inc. is a leading franchise specializing in biohazard and decontamination cleanup services. Founded in 2006 and headquartered in Greenwood Village, Colorado, Bio-One began franchising in 2010. 

The company focuses on providing essential services such as crime scene cleanup, hoarding remediation, and biohazard decontamination, addressing some of the most challenging and sensitive situations with discretion, care, and compassion. 

Bio-One differentiates itself by not only being the first franchise in this niche but also by maintaining a steadfast commitment to its mission of "Help First, Business Second."

Initial investment

The initial investment required for a Bio-One franchise is $116,000 - $170,000. That is the total cost you would need to finance if you were to start this franchise. These costs are provided by the franchisor in the Franchise Disclosure Document.

Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795

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Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795

Franchise Disclosure Document

Below is Bio-One's 2024 Franchise Disclosure Document. Upgrade to Pro or purchase the FDD to view and download the document.

Number of units

Bio-One had 130 total units in 2024, of which 130 were franchised-owned and 0 company-owned.

Frequently Asked Questions

Competitors

Below are some of

Bio-One

key competitors in the

Commercial Cleaning

sector.

Franchise
Franchisees
Royalty fee
Total investment
Revenue
Upgrade
EBITDA
Upgrade

130

New
+
8%
8%

7.50%

$116,000

-

$170,000

$423,000

n.a.

$xxx,xxx

n.a.

xx%

n.a.

Training 

Bio-One Franchise offers comprehensive training and support to ensure franchisees are well-prepared to operate successfully in the biohazard and decontamination cleanup industry. The training program is designed to equip franchisees with the necessary skills, knowledge, and resources to handle the sensitive and demanding nature of the work.

Initial Training

Bio-One provides an intensive initial training program at their corporate headquarters. This program typically lasts several days and covers a broad range of topics essential for running a Bio-One franchise. The initial training includes:

  1. Operational Procedures: Franchisees learn the standard operating procedures for biohazard cleanup, crime scene cleaning, hoarding remediation, and other decontamination services. This ensures that they can perform these tasks efficiently and safely.
  2. Safety Protocols: Given the nature of the work, safety is paramount. Bio-One trains franchisees on all necessary safety protocols, including the use of personal protective equipment (PPE), proper handling and disposal of hazardous materials, and adherence to OSHA guidelines.
  3. Customer Service: Handling emotionally sensitive situations is a core part of Bio-One’s services. The training includes strategies for interacting with clients compassionately and professionally during their most difficult times.
  4. Business Management: Franchisees are instructed on the business aspects of running a Bio-One franchise, including financial management, marketing strategies, and operational logistics. This helps ensure that franchisees are not only proficient in the cleanup services but also in managing their business effectively.

Territory Protection

Bio-One offers territory protection to its franchisees, ensuring that each franchisee has a defined and exclusive geographic area to operate within. This territorial exclusivity allows franchisees to develop their business without the concern of direct competition from other Bio-One franchisees in their immediate market. 

The protected territory is designed to provide enough potential clientele to support a thriving business, taking into account the population density and demand for biohazard cleanup services.

By offering this level of territorial protection, Bio-One helps franchisees establish a strong market presence and build lasting relationships with clients and local authorities. 

Competitors

Below are some of Bio-One's key competitors in the Commercial Cleaning sector.

Daigle Cleaning Systems

Franchisees

?

2
+
0%
0%
Investment

?

$54,000 - $68,000
Revenue

?

Upgrade

Summit Building Services

Franchisees

?

1
+
n.a.
n.a.
Investment

?

$26,000 - $46,000
Revenue

?

Upgrade

CLEANSTART SYSTEMS

Franchisees

?

2
+
0%
0%
Investment

?

$30,000 - $38,000
Revenue

?

Upgrade

Cleanest Restaurant Group

Franchisees

?

10
+
900%
900%
Investment

?

$103,000 - $144,000
Revenue

?

Upgrade

Cleannet

Franchisees

?

182
+
6%
6%
Investment

?

$20,000 - $85,000
Revenue

?

Upgrade

You've Got Maids

Franchisees

?

74
+
-15%
-15%
Investment

?

$36,000 - $107,000
Revenue

?

Upgrade

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