Bio-One Franchise FDD, Costs & Fees (2025)

KEY FRANCHISE STATS

All you need to know about this franchise in a snapshot

Franchisees

?

130
+
8%
8%
Investment required

?

$116,000 - $170,000
Revenue (AUV)

?

$423,000
Undisclosed
Pro
+
n.a.
+
xx%
-xx%
n.a.
Initial franchise fee

?

$60,000
Royalty fees

?

7.50%
+
2% + 5%
Operating Profit

?

n.a.

Pro
Franchisees

?

130
+
8%
8%
Investment required

?

$116,000 - $170,000
Franchise fee

?

$60,000
Royalty fees

?

7.50%
+
2% + 5%
Revenue (AUV)

?

$423,000
Undisclosed
Pro
+
n.a.
+
xx%
-xx%
n.a.
Operating Profit

?

n.a.

Pro

Bio-One: Leading the Way in Biohazard and Decontamination Cleanup

Bio-One Inc. is a leading franchise specializing in biohazard and decontamination cleanup services. Founded in 2006 and headquartered in Greenwood Village, Colorado, Bio-One began franchising in 2010. 

The company focuses on providing essential services such as crime scene cleanup, hoarding remediation, and biohazard decontamination, addressing some of the most challenging and sensitive situations with discretion, care, and compassion. 

Bio-One differentiates itself by not only being the first franchise in this niche but also by maintaining a steadfast commitment to its mission of "Help First, Business Second." This approach ensures that franchisees are trained to handle emotionally charged situations with professionalism and empathy, providing invaluable support to communities in distress.

Initial investment

Here's what you would need to invest if you were to start this franchise. These costs are provided by the franchisor in the Franchise Disclosure Document.

table { width: 100%; border-collapse: collapse; } table, th, td { border: 1px solid black; } th, td { padding: 8px; text-align: left; } th { background-color: #f2f2f2; }
Type of Expenditure Amount
IFF $60,000
QSP Package $39,500
Technology Startup Fee $1,595
Annual Conference Registration Deposit $1,000
Rent and Rental Improvements $0 to $600
Furniture, Fixtures, and Equipment $0 to $2,000
Computer Hardware, Software and Other Electronics $800 to $7,800
Initial Training Expenses $1,000 to $2,500
OSHA Training Fee $1,500
OSHA Training Expenses $1,000 to $2,500
Business Vehicle $0 to $30,000
Business Vehicle Tax, Title and License $0 to $1,500
Business Vehicle Insurance $150 to $300
Insurance and Professional Services $750 to $4,000
Additional Funds - 3 months $9,000 to $15,000
TOTAL $116,295 to $169,795

Franchise Disclosure Document

Upgrade to Pro to download this FDD

Access the most comprehensive franchise database

Upgrade to Pro
Thank you for signing up
Complete your upgrade now
Signup successful!
Complete your upgrade

Competitors

Franchise
Franchisees
Growth
Initial fee
Investment
Revenue
Gross Profit
Operating Profit
Industry

130

+
8%
8%
No growth
New

$60,000

$116,000

-

$170,000

n.a.

$423,000

Pro

n.a.

Pro

n.a.

Pro

Business Services

Training 

Bio-One Franchise offers comprehensive training and support to ensure franchisees are well-prepared to operate successfully in the biohazard and decontamination cleanup industry. The training program is designed to equip franchisees with the necessary skills, knowledge, and resources to handle the sensitive and demanding nature of the work.

Initial Training

Bio-One provides an intensive initial training program at their corporate headquarters. This program typically lasts several days and covers a broad range of topics essential for running a Bio-One franchise. The initial training includes:

  1. Operational Procedures: Franchisees learn the standard operating procedures for biohazard cleanup, crime scene cleaning, hoarding remediation, and other decontamination services. This ensures that they can perform these tasks efficiently and safely.
  2. Safety Protocols: Given the nature of the work, safety is paramount. Bio-One trains franchisees on all necessary safety protocols, including the use of personal protective equipment (PPE), proper handling and disposal of hazardous materials, and adherence to OSHA guidelines.
  3. Customer Service: Handling emotionally sensitive situations is a core part of Bio-One’s services. The training includes strategies for interacting with clients compassionately and professionally during their most difficult times.
  4. Business Management: Franchisees are instructed on the business aspects of running a Bio-One franchise, including financial management, marketing strategies, and operational logistics. This helps ensure that franchisees are not only proficient in the cleanup services but also in managing their business effectively.

Territory Protection

Bio-One offers territory protection to its franchisees, ensuring that each franchisee has a defined and exclusive geographic area to operate within. This territorial exclusivity allows franchisees to develop their business without the concern of direct competition from other Bio-One franchisees in their immediate market. 

The protected territory is designed to provide enough potential clientele to support a thriving business, taking into account the population density and demand for biohazard cleanup services.

By offering this level of territorial protection, Bio-One helps franchisees establish a strong market presence and build lasting relationships with clients and local authorities. 

Number of units

2024
Franchised units

120

128

130

Company-owned units

0

0

0

Total units

120

128

130

Competitors

Buildingstars

Investment required
$40,000
-
$53,000
Franchisees
1060
+
31%
31%

Window Gang

Investment required
$108,000
-
$149,000
Franchisees
48
+
7%
7%

Vanguard Cleaning Systems

Investment required
$148,000
-
$462,000
Franchisees
56
+
2%
2%

Two Maids

Investment required
$93,000
-
$150,000
Franchisees
117
+
29%
29%

Access the most comprehensive franchise database

For franchisees

Find the best franchises to invest into

For franchisors

Compare your brand to competitors

Get unlimited access to:
2,145 franchises data
Number of locations
Franchise fees
Total investment

?

Download unlimited FDDs
Revenue (AUV)

?

Revenue growth (last 3 years)

?

Profit metrics

?

Upgrade to Pro

Access the most comprehensive franchise database

For franchisees

Find the best franchises to invest into

For franchisors

Compare your brand to competitors

Upgrade to Pro
$180 / year
Total investment

?

Revenue (AUV)

?

Revenue growth (last 3 years)

?

Profit margin

?

Franchise Disclosure Documents
Advanced filtering and sorting
Watchlist

?

Priority support

?

Upgrade to Pro
Signup successful!
Complete your upgrade

A comprehensive and transparent look at franchising finances. The inclusion of profit margins and disclosure documents offers insights you can’t find elsewhere. Essential for anyone considering a franchise investment.

Darren M.

Franchisee

Been waiting for years for this. All the most important franchises and their latest Franchise Documents to download. The team also updates the franchises very quickly.

Emily V.

Franchisee