
Bio-One Inc. is a leading franchise specializing in biohazard and decontamination cleanup services. Founded in 2006 and headquartered in Greenwood Village, Colorado, Bio-One began franchising in 2010.
The company focuses on providing essential services such as crime scene cleanup, hoarding remediation, and biohazard decontamination, addressing some of the most challenging and sensitive situations with discretion, care, and compassion.
Bio-One differentiates itself by not only being the first franchise in this niche but also by maintaining a steadfast commitment to its mission of "Help First, Business Second."
Here's what you would need to invest if you were to start this franchise. These costs are provided by the franchisor in the Franchise Disclosure Document.
Bio-One Franchise offers comprehensive training and support to ensure franchisees are well-prepared to operate successfully in the biohazard and decontamination cleanup industry. The training program is designed to equip franchisees with the necessary skills, knowledge, and resources to handle the sensitive and demanding nature of the work.
Bio-One provides an intensive initial training program at their corporate headquarters. This program typically lasts several days and covers a broad range of topics essential for running a Bio-One franchise. The initial training includes:
Bio-One offers territory protection to its franchisees, ensuring that each franchisee has a defined and exclusive geographic area to operate within. This territorial exclusivity allows franchisees to develop their business without the concern of direct competition from other Bio-One franchisees in their immediate market.
The protected territory is designed to provide enough potential clientele to support a thriving business, taking into account the population density and demand for biohazard cleanup services.
By offering this level of territorial protection, Bio-One helps franchisees establish a strong market presence and build lasting relationships with clients and local authorities.
Below are some of
Bio-One
key competitors in the
Commercial Cleaning
sector.

130
$60,000
$116,000
$170,000
n.a.
$423,000
$xxx,xxx
n.a.
n.a.
n.a.
n.a.

Cleanest Restaurant Group is a restaurant cleaning service franchise offering specialized front- and back-of-house deep cleaning, serving full-service and quick-service restaurants, and known for niche expertise, proprietary cleaning solutions, low-competition territories, and recurring nighttime service contracts.
?
?

CleanNet is a commercial cleaning franchise offering janitorial and facility-maintenance services to offices and industrial properties, serving business clients and operators, and known for proprietary systems, national account support, and scalable operations.
?
?

You've Got Maids is a residential cleaning franchise providing professional maid services with a focus on eco-friendly practices and exceptional customer service.
?
?

Renue is a commercial cleaning and sanitization franchise specializing in deep cleaning and surface restoration for hotels, healthcare facilities, and offices.
?
?

Buildingstars provides commercial cleaning and janitorial services, focusing on quality and customer satisfaction to maintain clean and professional workspaces.
?
?

Sparkle Squad provides residential and commercial window cleaning services. Their eco-friendly solutions and professional techniques leave windows spotless and sparkling.
?
?
Choosing a franchise goes beyond reviewing fees and financials — it requires an honest assessment of your goals, skills, and risk tolerance. This article outlines the key questions prospective owners should ask before committing. It helps clarify whether the opportunity aligns with your lifestyle, long-term plans, and operational preferences.
Speaking with current and former franchisees is one of the most reliable ways to understand how a franchise really operates. This guide explains how to conduct validation calls, what questions to ask, and how to identify patterns in feedback. It provides a practical framework for uncovering real performance expectations and support quality.
Not all franchise opportunities offer the same potential. This article breaks down how to evaluate a brand’s industry, financial health, support systems, and competitive positioning. It helps prospective buyers compare options and identify franchises with strong long-term viability.
A clear, well-structured business plan is essential for securing financing and preparing for the realities of franchise ownership. This article explains how to build a plan that covers market analysis, operations, staffing, and local marketing. It also outlines how to create realistic pro forma financials, including revenue projections, startup costs, and break-even analysis. Readers gain a practical framework they can use when applying for loans or planning their first years of operations.